Mesothelin anticorps (AA 273-407) (CF®568)

N° du produit ABIN7656962

L’anticorps Souris Monoclonal anti-Mesothelin a été validé pour IHC et IHC (fp). Il convient pour détecter Mesothelin dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Mesothelin anticorps (AA 273-407) (CF®568) (ABIN7656962)

Antigène: Mesothelin (MSLN)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Mesothelin est conjugé à/à la CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MSLN-2131

Détail du produit anti-Mesothelin anticorps (CF®568)

Épitope: AA 273-407

Fonction: Mesothelin (Mesothelial Marker) (MSLN/2131), CF568 conjugate

Attributs du produit: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Mesothelin

Antigène: Mesothelin (MSLN)

Autre désignation: Mesothelin

Sujet:

Synonyms: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP

Gene Symbol: MSLN

Poids moléculaire: 40 kDa (Mature form), 69 kDa (Precursor)

ID gène: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process